western blotting of acidic proteins

[Dr. Karthikeyan Narayanan]

Dear Netters;
I have problem in transfering the acidic proteins (usually found in
dentin) or phosphophoryns onto a nitrocellulose membrane. Can any one
give me suggestions on this.
Thankx in advance
--
********************************************************************************************

Narayanan Karthikeyan Ph.D.,
College of Dentistry,
Department of Oral Biology,
University of Illinois at Chicago
Chicago IL 60612
Tel: 312-413-5375
Fax: 312-413-6694
********************************************************************************************




---