western blotting of acidic proteins

Chris LaRosa clarosa at biocomp.unl.edu
Wed Jun 14 18:54:14 EST 2000



"Dr. Karthikeyan Narayanan" wrote:
> 
> Dear Netters;
> I have problem in transfering the acidic proteins (usually found in
> dentin) or phosphophoryns onto a nitrocellulose membrane. Can any one
> give me suggestions on this.
> Thankx in advance
> --
> 
Try PVDF.  Try reversing polarity.






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