Who is wrong with primer design?

Chris LaRosa clarosa at biocomp.unl.edu
Thu Jun 15 10:54:42 EST 2000


pretty sure that in the case of cycle sequencing primers a study was
> run (published in Biotechniques?) in which every primer possible for a
> particular sequence was synthesised and tested. From memory what
> appeared to be poor primers (from the design point) worked no worse than
> good primers.
> 
> 
So are you suggesting that primer design programs like oligo give false
promise of good primer design?   As I recall others have questioned the
algorithms uses in Oligo.






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