lacZ minus pET expression strain?

Rafael Maldonado rmaldonado at
Mon Jun 19 12:49:53 EST 2000

There are several strains with that genotype. Check the catalogs of the
companies selling the DE3 system, as novagen ( Also,
they sell the DE3 phage, so you can lisogenize the strain you want to
express the T7 polymerase in.

dalex at wrote:
> I am in search of a lacZ minus strain of E. coli
> that is a lysogen for the DE3/T7 polymerase system
> (and therefore can be used for pET expression)

Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)

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