lacZ minus pET expression strain?

Rafael Maldonado rmaldonado at ua.es
Mon Jun 19 12:49:53 EST 2000


There are several strains with that genotype. Check the catalogs of the
companies selling the DE3 system, as novagen (www.novagen.com). Also,
they sell the DE3 phage, so you can lisogenize the strain you want to
express the T7 polymerase in.

dalex at nexus.microimm.mcgill.ca wrote:
> 
> I am in search of a lacZ minus strain of E. coli
> that is a lysogen for the DE3/T7 polymerase system
> (and therefore can be used for pET expression)
>

-- 
Rafael Maldonado
Divison of Genetics
University of Alicante (Spain)






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