Help with Taxol preps

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Tue Jun 20 16:30:22 EST 2000


Ian, 

maybe it is helpful when the stock is diluted into FCS or albumin and 
then into medium. this procedure was successful for me when using 
squalene and is reported for some lipids (Pagano et.al., somewhere in 
the late 80's IIRC)

wolfgang

> From:          Ian Harper <Ian.Harper at sci.monash.edu.au>
> Subject:       Help with Taxol preps
> Date:          Tue, 20 Jun 2000 23:13:48 +1000
> Organization:  Monash Uni
> To:            cellbiol at hgmp.mrc.ac.uk, cytonet at hgmp.mrc.ac.uk, methods at hgmp.mrc.ac.uk,
>                proteins at hgmp.mrc.ac.uk

> Hi,
> A student of ours is using Taxol to disrupt the cytoskeleton, and
> has started getting unexpected preciptations forming in the medium
> after switching from a pharmaceutical grade to the "sigma" powder. 
> A stock is made up either in DMSO or Cremophor El (oil based I
> think) + Ethanol ( as suggested by supplier), but when the stock is
> diluted 0.25% vol to the aqueous, we see needle-shaped crystal
> forming as taxol goes out of solution. Ideas, and protocols would be
> greatly appreciated, plz email to me: Ian.Harper at sci.monash.edu.au
> 
> Thanks
> 
> Ian.
> 
> ><O><1><O><1><O><1><O><1><O><1><O><1><O><1><
> Ian Harper
> Confocal Imaging Facility
> Dept Biological Sciences
> Monash University
> Wellington Rd, Clayton
> VIC, Australia
> Tel: +61-3-99055635, Fax: +61-3-99055613
> Email: Ian.Harper at sci.monash.edu.au
> ><O><1><O><1><O><1><O><1><O><1><O><1><O><1><
> 
> 
> 
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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