cereal transformation

C Hanns cwh93 at hotmail.com
Thu Jun 22 02:35:58 EST 2000


I'm transforming various cereals but I am having difficulty identifying true 
transgenics - not sure if my transformations are failing or if there's a 
problem with the DNA I'm isolating etc (PCR for the transgene works fine 
from the plasmid. Can anyone suggest a low/single copy nuclear gene or 
primer pairs (preferably fairly conserved between several cereal species) 
which I can use as an internal positive control for my PCR?  Any help is be 
much appreciated.


Claire


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