Restriction digest problem

chihara chihara at usfca.edu
Thu Jun 22 23:59:29 EST 2000


We are having a problem digesting two P1 vectors with inserts.  We got a
set of seven, and had no trouble with 5. But two of the minipreps when
digested with EcoR1 or HindIII seem to break down and give nothing but a
big smear.
We have repeated this several times with the same results.  We see DNA
and get a positive Southern blot with our probe, so we know the DNA is
there.
Any suggestions as to what to try?



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