PCR from polyacrylamide gel slice
clarosa at biocomp.unl.edu
Fri Jun 23 09:19:51 EST 2000
> Is it possible to run a PCR reaction using a slice of the polyacrylamide gel
> that has my band of interest? Or do I need to get the DNA out of the gel
> first? Thanks.
Try the old freezing crushing and soaking, method which can be followed
by an number of standard clean up procedures (phenol, chloroform) or
optional no cleanup.
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