PCR from polyacrylamide gel slice

Chris LaRosa clarosa at biocomp.unl.edu
Fri Jun 23 09:19:51 EST 2000



Gary wrote:
> 
> Is it possible to run a PCR reaction using a slice of the polyacrylamide gel
> that has my band of interest?  Or do I need to get the DNA out of the gel
> first?  Thanks.
> 
> GAry

Try the old freezing crushing and soaking, method which can be followed
by an number of standard clean up procedures (phenol, chloroform) or
optional no cleanup.






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