PCR from polyacrylamide gel slice

Chris LaRosa clarosa at biocomp.unl.edu
Fri Jun 23 09:20:59 EST 2000



Gary wrote:
> 
> Is it possible to run a PCR reaction using a slice of the polyacrylamide gel
> that has my band of interest?  Or do I need to get the DNA out of the gel
> first?  Thanks.
> 
> GAry

You can also electroelute out the dna.  For instance, Biorad and other
electroeluters.  You might be able to easily download their manuals and
protocols to get an idea.






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