ppt'n of RNA from sucrose gradient--isopropanol or ethanol??
mdep at musica.mcgill.ca
Fri Jun 23 19:52:09 EST 2000
I am isolating RNA from sucrose gradients of polysome profiles. I
pheno/chloroform extract twice, then 1 time with chloroform and then
precipitate with 0.85 volumes of isopropanol. Recently, I used phenol
buffered with citrate at pH 4.3 and had poor recovery. I went back to
water buffered-phenol and had recovery. However, I think that using
isopropanol is not efficient enough. Do you guys think that I should
precipitate using salt and 2.5 vol ethanol instead?? Thanks.
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