PCR from polyacrylamide gel slice

Liz Parks liz_parks at ncsu.edu
Fri Jun 23 12:46:16 EST 2000


Hi Gary,
I have had much success using a method published in Biotechniques
(vol.17, no.5, p.914).  Here's the protocol in a nutshell.  Cut out the
band with a sterile scalpel.  Incubate it at 95C for 20 minutes in 100ul
of 10mM Tris-HCl, 50mM KCl, 1.5mM MgCl2, 0.1% Triton X-100, pH 9.0.  Use
1ul in your PCR reaction.  You can store the unused portion at -20C and
reuse it.
Good luck!

Liz

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Liz Parks
North Carolina State University
Department of Plant Pathology
Box 7616
Raleigh, NC  27695

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