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Peter Pediaditakis pxpst2 at dlt.pitt.edu
Mon Jun 26 07:16:26 EST 2000


In article
<Pine.LNX.4.21.0006232016410.12942-100000 at mailhost.tifr.res.in>,
hsatreya at tifr.res.in (Atreya Hanudatta Sas) wrote:

>   Has anybody experienced that his/her protein gets denatured or
> unfolded upon lyophilizing ? If so, what may be the reason for the same. I
> tried lyophilizing my protein, but it seems to get unfolded. How should I
> concentrate my protein sample in that case ? 
>         

Are you certain that your sample is frozen during the lyophilization
process?  Many peaple think they are lyophilizing but in actuality, they
are not subliming the sample, they are evaporating the sample.

-- 
Peter Pediaditakis
University of Pittsburgh
Dept. of Pathology

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