Quantitating Westerns

Chris LaRosa clarosa at biocomp.unl.edu
Mon Jun 26 08:58:59 EST 2000


> CCD camerra, you can extend your linear range by one order of magnitude on
> the low end and on the high end.
> 
> regards
> Peter Pediaditakis

Using an appropriate std curve, one may also use slot blot westerns and
scan.   One may use I125 labeled antibodies and scan films, or one may
use chemical methods, and I suppose chemiluninescence.  You have to run
a standard curve on each blot.






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