Query on Nickel Affinity Columns
jkuruvil at lamar.colostate.edu
Tue Jun 27 14:56:52 EST 2000
Thankyou!!!! It really works...!!!!!!!!!
"Dr. Duncan Clark" wrote:
> In article <3957AC16.F910A81 at lamar.colostate.edu>, jkuruvil
> <jkuruvil at lamar.colostate.edu> writes
> >Does anybody know of a good scource for Nickel Affinity columns...and a
> >GOOD BUFFER that can be used to regenerate them????
> We are having a some good results with IDA-sepharose (Sigma or APB). You
> can strip the Nickel from the column after each run with 25mM EDTA and
> then wash the column with 0.1M NaOH prior to equilibrating back in
> water/buffer and re-charging with fresh Ni (in our case we use Nickel
> sulphate, rather than Chloride 'cos I had a kg on the shelf!).
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....
> Duncan Clark
> DNAmp Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382
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