High background in Southern hybridization

Mikko Taipale mikko.taipale at helsinki.fi
Wed Jun 28 10:29:21 EST 2000


Roland Hübner <rhubner at gins.uia.ac.be> wrote:
>> I've had annoying problems with high background in standard Southern
>> hybridization. 

> Hello,
>  what membrane do you use for this job?
> R.

I use charged Hybond N+, but I crosslink the DNA with UV, nevertheless.

Mikko






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