High background in Southern hybridization

R. John Lye rjl6n at virginia.edu
Thu Jun 29 06:13:38 EST 2000

Mikko Taipale wrote:

> Do you also use charged nylon

Lately I've been using Hybond XL.

> and alkaline blotting?


> With positively
> charged membranes, I believe there is no need for additional crosslinking
> provided that the blotting is done with an alkaline solution.

That's probably true, but I still bake them anyway (after neutralization).
Its the UV crosslinking that seemed to cause the background problems;
baking doesn't seem to do that (in my hands anyway).


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