Best blocking reagent ?

Svend Kjaer svend at cajal.mbb.ki.se
Thu Jun 29 07:44:30 EST 2000


Dear all,

I would like to hear of some with alternative suggestions for efficient
blocking reagents for ELISA.
Most often I used skimmed milk powder in PBS and that works like a charm
with a very low background. Alternatively, I go for BSA, but that gives
a somewhat higher background.
I would like to have a very low background, yet avoid the use of milk
powder, which contains non-defined amounts of Calcium as the ELSIA
supposedly assay is calcium-dependent
Therefore, are there any alternatives to BSA in providing good blocking
of non-specific binding ??

Thanx,

/svend

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Svend Kjær, Ph.D.
Division of Molecular Neurobiology,
Department of Neuroscience
Karolinska Institute
Berzelius väg 1, 3rd floor
171 77 Stockholm
Sweden

Tel: +46-8-7287666
Fax: +46-8-339548

Private phone: +46-8-6409962
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