Internal RT-PCR controls
Dr. Duncan Clark
drc at nospam.demon.co.uk
Thu Jun 29 10:46:39 EST 2000
In article <395B2FFF.DDF600F8 at sheffield.ac.uk>, Lee Hunt
<L.hunt at sheffield.ac.uk> writes
>Can I use my internal control primers in the same PCR reaction as my
Yes solely providing the primer pairs do not interact.
>and is this more quantitiative than doing the 2
Probably, but only if the primer pairs amplify their targets with the
same efficiencies and that one primer pair does not out compete the
second pair for reagents. That would mean that the product bands for
each target should appear at similar cycle numbers. A bit of work will
need doing to check this out before you rely on the result.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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