High background in Southern hybridization

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Fri Jun 30 01:18:03 EST 2000

"R. John Lye" wrote:

> I've gotten much better results by switching to the "Modified Church
> and Gilbert Buffer" that Amersham recommends for Hybond
> (0.5 M NaPO4 pH 7.2, 7% SDS (w/v), 10 mM EDTA) - and it is much
> simpler to make up, as well.

Is this the stuff Boehringer sells as Easy Hyb? It must be a high SDS buffer,
but it seems to me (my nose) that there is ammonia in it. Does anyone have a
recipe? Has anyone used home-made high SDS for Northerns?

Susanne Rohrer
Institute of Medical Microbiology
University of Zurich

srohrer at immv dot unizh dot ch

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