pcr from acrylamide gel slice

Mr JN Goulding jgouldin at hgmp.mrc.ac.uk
Fri Jun 30 08:29:44 EST 2000


Yes it is possible to do PCR by just bunging the acrylamide gel slice into the pcr mix. Works well, and saves a lot of time and messing about eluting it. Basically i just added the gel slice to the pcr mix (100ul volume) and amplified as normal. Got very good results. Jon.






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