microarrays

Karthik Aghoram kaghoram at unity.ncsu.edu
Thu Jun 29 12:33:21 EST 2000


Hi,

Microarrays are the new rage in transcript expression studies.  The 
biggest advantage is that one may study the expression of 1000s of genes 
simultaneously.  In short, it is a high-throughput Northern.  

In brief, tiny either cDNAs or synthetic oligonucleotides (representing 
unique sequences) are immobilized in an array on a glass slide or a 
"micro-chip."   1000s of such sequences (e.g. the entire yeast genome) 
can be arrayed on a single chip.

RNA isolated from tissues treated under various conditions is transcribed
and labeled with a unique fluorescent dye.  That is, each RNA sample is
labeled with a dye that fluoresces at a particular wavelength (say control
samples are labeled red and treated with green).  The labeled RNA is
hybridized to the DNAs on the slide or chip.  The fluorescence is then
imaged. 

The ratio of red/green at a particular spot is a measure of the ratio of
the relative expression of the sequence that was placed on that spot in
the slide.  So one could get a comprehensive profile of gene-expression 
in one shot.

Hope this helps.

Karthik

T V Naga Rama Chander (chander at kelvin.ncl.res.in) wrote:
: 
: dear all,
: i w'd like u tell me what is/are microarray(s) all about.
: where are they used.
: thanx,
: 
: ramchander.
: plant tissue culture division	     !
: national chemical laboratory	     !
: pune-411008.			     !
: india.				     !
: tel: 91-20-5893300 ext: 2219         !
: fax: 91-20-5893438		     !
: e-mail: chander at kelvin.ncl.res.in    !
: 
: 
: ---

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Karthik Aghoram				Postdoctoral Associate
					Dept of Crop Science
e-mail: kaghoram at unity.ncsu.edu		North Carolina St University
Phone (W): 919-515-2705			Raleigh, NC 27695-7620

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