a-P-Y Western with PBST??

Martin Offterdinger martin.offterdinger at akh-wien.ac.at
Wed Mar 1 05:51:05 EST 2000


Hi
We are currently facing serious problems using a-PY Westerns; we cannot
detect our activated receptor-tyrosine kinase.
I have found a hint that only TBST must be used for P-Y.
Is there anyone who can confirm this statement?
A P-Y antibody should detect phosphotyrosine and not inorganic phosphate,
isn't it?
But I have tried both 4G10 and PY20 without success.
Is there a general recommendation which is the best available AB for
Westerns?
There is also a mixture of monoclonals available from Zymed.
Are there any experiences with it?
Thank you!
Martin






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