western blot: colorless protein bands

Andrew Leung s970854 at cuhk.edu.hk
Wed Mar 1 09:46:31 EST 2000


I have this experience before. But I can't explain.

ITABAJARA DA SILVA VAZ JUNIOR ¼¶¼g©ó¤å³¹
<01JMHKHPYAJ60002N2 at if1.if.ufrgs.br>...
Dear all,
I am working with Western-blot.
Hello there,
In a Western blot the nitrocellulose was blocked with 5% blotto
and an anti-mouse IgG-phosphatase alkaline conjugate was
used.
These produce a negative image of colorless protein bands
against a clear background.
Any help will be appreciated.
__________________________________________________
Itabajara da Silva Vaz Junior
Faculdade de Veterinaria e
Centro de Biotecnologia do RS
Universidade Federal do Rio Grande do Sul
Av. Bento Goncalves 9500   C.P. 15005
Predio 43421 - Campus do Vale
91501-970  Porto Alegre RS   Brasil
Fone (51) 3166078    Fax (51)319-10-79  [00 55 51 3191079]
E-mail: ITA at DNA.CBIOT.UFRGS.BR
http://www.ufrgs.br/depbiot/201/cbt201.htm
http://www.ufrgs.br/favet/imunovet
---






More information about the Methods mailing list