low copy plasmid isolation

Jay T. Myers jtmyers at umich.edu
Wed Mar 1 09:56:09 EST 2000


Just noted this posting.  I don't have any experience with pSC101 so I can't
comment as to whether or not amplification will work in this case.  Dr.
Clark is right you may have no other choice but to maxi-prep till the cows
come home.  Ouch!

> From: "Dr. Duncan Clark" <Duncan at nospam.demon.co.uk>
> Organization: DNAmp Ltd.
> Reply-To: "Dr. Duncan Clark" <Duncan at genesys.demon.co.uk>
> Newsgroups: bionet.molbio.methds-reagnts
> Date: Tue, 29 Feb 2000 11:10:16 +0000
> Subject: Re: low copy plasmid isolation
> 
> In article <89ep5p$k5k$1 at newsflash.concordia.ca>, ELAINE B S NEWMAN
> <neweb at alcor.concordia.ca> writes
>> Can anyone tell me how to get enough plasmid from a cell containing a psc101
>> plasmid in order to cut it and check plasmid structure?
> 
> No easy way other than growing up lots and lots. I don't think the
> replicon in pSC101 is amplifiable. Anyone know for sure?
> 
> Duncan
> -- 
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....
> 
> Duncan Clark
> DNAmp Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382
> http://www.dnamp.com
> http://www.genesys.demon.co.uk





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