sephadex problem

Chris LaRosa clarosa at biocomp.unl.edu
Wed Mar 1 10:37:53 EST 2000


>
> > The course is probably ok for larger chromatographic columns or batch
> > purifications.  The Course means larger beads.   For mini spin columns
> > finer is probably better.
>
> Batch purification in gel filtration chromatograpy? What do you mean?
>
> If your desalting you dont need to run a column.  You just had your protein
> solution to a beaker of G25, and decant or filter it.  The small molecules
> are retained in the beads.    If your trying to separate proteins then you
> will need a column.





More information about the Methods mailing list