sephadex problem

[Chris LaRosa]

>
> > The course is probably ok for larger chromatographic columns or batch
> > purifications.  The Course means larger beads.   For mini spin columns
> > finer is probably better.
>
> Batch purification in gel filtration chromatograpy? What do you mean?
>
> If your desalting you dont need to run a column.  You just had your protein
> solution to a beaker of G25, and decant or filter it.  The small molecules
> are retained in the beads.    If your trying to separate proteins then you
> will need a column.