Paul S. Brookes.
brookes at uab.edu
Wed Mar 1 13:19:17 EST 2000
Sigma also sells a precursor - G3P diethylacetal (G5376), from which you
can make a solution of G3P relatively easily - it's also cheaper and keeps
for longer in the freezer.
If you require it for a GAPDH assay, don't forget you can also assay this
enzyme backwards using 3PG as substrate with PGK as auxilliary enzyme and
follow NADH going the other way. I have protocols for both the forward and
backward assays if you need them.
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
The quality of e-mails can go down as well as up
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