cloning the wrong way?

Chris LaRosa plarosa1 at navix.net
Thu Mar 2 06:21:44 EST 2000


Alternatively,,,,use  error correcting polymerases.


"Richard P. Grant" wrote:

> In article <38AAD3B4.D212C32C at uni-konstanz.de>,
> Frank.Fackelmayer at uni-konstanz.de wrote:
>
> > ALWAYS!!! Especially when using Taq polymerase which is notorious for
> > making mistakes.
>
> Pish.
>
> To the second part of this, anyway.  Drop the dNTP conc to 50 uM (from
> 200 uM) and watch your error rate fall away.
>
> R
>
> --
> Richard P. Grant MA DPhil
> Structural Studies Group, MRC-LMB
> http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
> Please reply to rpg 'at' mrc-lmb.cam.ac.uk





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