cloning the wrong way?
plarosa1 at navix.net
Thu Mar 2 06:21:44 EST 2000
Alternatively,,,,use error correcting polymerases.
"Richard P. Grant" wrote:
> In article <38AAD3B4.D212C32C at uni-konstanz.de>,
> Frank.Fackelmayer at uni-konstanz.de wrote:
> > ALWAYS!!! Especially when using Taq polymerase which is notorious for
> > making mistakes.
> To the second part of this, anyway. Drop the dNTP conc to 50 uM (from
> 200 uM) and watch your error rate fall away.
> Richard P. Grant MA DPhil
> Structural Studies Group, MRC-LMB
> Please reply to rpg 'at' mrc-lmb.cam.ac.uk
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