Fuzzy PCR product band

AlPiEs alpies at aol.com
Fri Mar 3 09:38:10 EST 2000


We are constructing a phage-based peptide library and have amplified a 123 b
oligonucleotide containing a 90 b randomized sequence using PCR.   When we run
the product in a 1% agarose gel we get a "fuzzy" band, in the 200-300 bp range.
 Is this normal? I thought that it might be due to the heterogeneity of the
product. Thanks. 
Alan Escher




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