Fuzzy PCR product band
jens at tornoe.net
Fri Mar 3 17:37:40 EST 2000
I have made the same observation after building dsDNA from two ~150 base
randomized oligos with complemetary 3' ends using Taq. The resulting band
appeared to be approximately the right size, but even if I didn't add Taq, a
band could still be seen on the gel. It was fainter, but had the same size.
All bands were very fuzzy. Column purification helped a bit.
We really couldn't explain what we saw, but since the synthesized dsDNA
could be cloned and the sequences were correct, I hurried on with the work
trying my best to forget that.
AlPiEs <alpies at aol.com> wrote in message
news:20000303093810.10719.00003200 at ng-da1.aol.com...
> We are constructing a phage-based peptide library and have amplified a 123
> oligonucleotide containing a 90 b randomized sequence using PCR. When we
> the product in a 1% agarose gel we get a "fuzzy" band, in the 200-300 bp
> Is this normal? I thought that it might be due to the heterogeneity of
> product. Thanks.
> Alan Escher
More information about the Methods