BSA in PCR

Chris LaRosa clarosa at biocomp.unl.edu
Fri Mar 3 18:27:38 EST 2000


The BSA works in part by stablizing the taq polymerase.  Protein
biochemist learned from experience that for some reasons still mysterious
to me,  proteins become unstable or tend to denature in solution when in
low concentration.  So if your trying to stablize an enzyme activity
during purification you might add bsa to stablize the enzyme in its active
form.   I am not sure of the actual mechanisms involved.

PCPhD

"R. Burkert" wrote:

> I use BSA as reagent in PCR, as a lot of other people do as well,
> but can anybody help me out finding a paper where the use is described
>





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