Trivial transformation improvement

Dr. Peter Gegenheimer PGegen at UKans.nolospamare.edu
Sat Mar 4 00:01:26 EST 2000


On Fri, 25 Feb 2000 19:12:55, gruther at bilbo.bio.purdue.edu (George Rutherford)
wrote:

ð You may be seeing a doubling of colonies over your time course, but do you
ð know whether or not you are seeing a doubling of identical sibs or might
ð they actually be unique clones? 

IF the cells are actually growing, you are probably seeing replication of 
transformed cells. This is OK unless you are trying to determine the 
representation of sequences in the original ligation mix, or are otherwise 
looking for independent transformants! 

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