cloning the wrong way?

[Richard P. Grant]

In article <8a0d0g$3cs$1 at scotsman.ed.ac.uk>, Chris Boyd 
<cboyd at holyrood.ed.ac.uk> wrote:

> ALWAYS sequence inserts that have been PCR cloned.

Chris,

I'm not disagreeing with that.  I just haven't seen the error rates that 
people claim with Taq.  But I always sequence, regardless (and yes, the 
biggest source of error does seem to be primer synthesis).

R

-- 
Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
http://www2.mrc-lmb.cam.ac.uk/personal/rpg/index.html
Please reply to rpg 'at' mrc-lmb.cam.ac.uk