cloning the wrong way?

Richard P. Grant rgrant at
Mon Mar 6 09:53:20 EST 2000

In article <8a0d0g$3cs$1 at>, Chris Boyd 
<cboyd at> wrote:

> ALWAYS sequence inserts that have been PCR cloned.


I'm not disagreeing with that.  I just haven't seen the error rates that 
people claim with Taq.  But I always sequence, regardless (and yes, the 
biggest source of error does seem to be primer synthesis).


Richard P. Grant MA DPhil          
Structural Studies Group, MRC-LMB
Please reply to rpg 'at'

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