cloning the wrong way?
Richard P. Grant
rgrant at netscape.net
Mon Mar 6 09:53:20 EST 2000
In article <8a0d0g$3cs$1 at scotsman.ed.ac.uk>, Chris Boyd
<cboyd at holyrood.ed.ac.uk> wrote:
> ALWAYS sequence inserts that have been PCR cloned.
I'm not disagreeing with that. I just haven't seen the error rates that
people claim with Taq. But I always sequence, regardless (and yes, the
biggest source of error does seem to be primer synthesis).
Richard P. Grant MA DPhil
Structural Studies Group, MRC-LMB
Please reply to rpg 'at' mrc-lmb.cam.ac.uk
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