NH4Cl - what is it good for?

Anton Tutter atutter at aim.salk.edu
Wed Mar 8 01:19:31 EST 2000


> What purpose does NH4Cl serve in DNase-treated solutions.
> Is it that it inhibits another activity of the enzyme?
> I'd appreciate some help with this matter..
 

it may also serve to selectively precipitate the treated DNA and not RNA,
which may be present in the DNase reactions, depending on how the reactions
were done (i.e., genomic DNA footprints, or with extracts, both of which
will have RNA that can interefere with the electrophoresis step).

--anton

tutter at mac.com





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