POLYRIBOSOMES

Mano mdep at musica.mcgill.ca
Thu Mar 9 18:36:17 EST 2000


Sorry, I was not clear..
The reason why I asked is because a collegue of mine vortexed her sample
and she got no polysomes on her profile.... I was wondering if vortexing
was the reason why her experiment failed??
Thanks,
Mano.

Nick Theodorakis wrote:

> In article <38C71533.FD7EAEAC at musica.mcgill.ca>,
>   Mano <mdep at musica.mcgill.ca> wrote:
> > Hi experts,
> >     Is vortexing of a post-nuclear extract enough to disrupt
> > polyribosomes such that they will not appear in a sucrose gradient?
> >
> > Thanks,
> > Mano.
> >
> >
>
> No.
>
> You can try EDTA or RNase to disrupt polysomes.
>
> --
>  _______________________________________________
> | Nick Theodorakis                              |
> | nicholas_theodorakis at urmc.rochester.edu       |
> |_______________________________________________|
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.





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