E. coli expressing taq pol

Chris LaRosa clarosa at biocomp.unl.edu
Mon Mar 13 17:14:11 EST 2000



Ron Hart wrote:

> I've been asking around for one of the E. coli/taq polymerase expressors,
> but so far no luck.  I'm about to start a large project amplifying thousands
> of clones, and I'd love to use "homemade" taq to save a few bucks.

We did what you want to do.    The resulting taq is good for routine stuff, but
it was highly contaminated with Ecoli dna, enough to give artifactual products
if one is not careful.   Unfortunately we never froze down the cells after
generating a lifetime supply of Taq polymerase.






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