Anti GAL4 DBD Antibody

cathy.slack at ic.ac.uk cathy.slack at ic.ac.uk
Wed Mar 15 11:29:15 EST 2000


Has anyone used the Santa Cruz anti GAL4 DNA binding domain monoclonal
primary antibody? I am using it in western blotting to detect expression
of a fusion protein for use in the yeast two hybrid system. I constantly
get non-specific binding to multiple proteins in untransformed yeast and
was wondering if anyone else has had the same problem.
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