screening lb agar plate for bacteria expressing a protein

Chris LaRosa clarosa at biocomp.unl.edu
Fri Mar 17 15:10:02 EST 2000



Chris LaRosa wrote:
> 
> See Sambrook et al chapter 12.24.  Screening expression libraries with
> antibodies and oligonucleotides.
> 
> I missed the GFP part..... We never did it but they say that if you have wild type GFP you can use a long wavelength UV hand held lamp to see fluorescent colonies on some constructs.    If your using Red shifted GFP (excitation wavelength 588) I dont know if the UV will work.  I am working with red shifted gfp but we can screen the cells on an inverted fluorescent microscope.  I suppose if you had one you could do that also with plates or filters but never tried it.




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