yeast two hybrid how long for clones to come out?

Koi Yau Lam kokoro at edsamail.com.sg
Sun Mar 19 04:52:48 EST 2000


I am using clontech's two hybrid system 
currently doing a high stringency selection ( - AHLT )
have been waiting for 10 days already about 12 clones have surfaced but all are false positives
anybody has any idea how long should I wait usually before I can say that there are no interactions?
PS this is a mini project for undergrads so I don't have time to wait too long hence this posting 
Thanks 
Kevin

---




More information about the Methods mailing list