identifying the protein in two hybrid studies

Philipp Pagel pagel at abraxas.magic.lan
Sun Mar 19 10:34:47 EST 2000

"Koi Yau Lam" <kokoro at> wrote:
> I am screening a cDNA Library for interactions and I plan to PCR sequence the insert to identify the protein 
> Does anybody has any experience with this? I need to know if the plasmid DNA obtained is pure enough or in sufficent quantities to directly sequence it with out amplifying with PCR 

We usually prepare DNA from the yeast clones and transform E. coli with
that to get clean DNA for sequencing. The DNA that you get directly from
yeast is not good enough.


Dr. Philipp Pagel
Cellular and Molecular Physiology         phone: (203) 785-6835	
Yale University
333 Cedar ST
New Haven, CT 06520

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