extinction coefficent ?

Chris LaRosa clarosa at biocomp.unl.edu
Mon Mar 20 13:22:20 EST 2000



Patrick D Duncan wrote:
> 
> Hi,
> Could someone tell me about the extinction coefficent?
> What is it and how does it relate to the Biuret Reaction?

For a particular wavelength in a spectometer, 

A= E x L x [C]   Beer-Lambert equation where E is the derived molar
extinction coefficient of a compound in solution, L (1 cm by convention)
is the path length, C is the Molar concentration   , and A is the
Absorbance.




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