PCR elongation time?
rh at mblab.gla.ac.uk
Mon Mar 20 12:55:27 EST 2000
In article <38D64A17.85FA247 at jouy.inra.fr>, Sophie Launey
<Sophie.Launey at jouy.inra.fr> wrote:
> Hello everybody,
> My question is: how short can the elongation time in a PCR cycle be?
> (for fragments of less than 300 bp).
Depends on your Thermal Cycler. On a RapidCycler which is Capiliary based.
you would only need to elongate for about 5 seconds maximum. SEE :-
Dependiong on the Taq ~ 50-90 bases/sec (roughly)
Bob, Sunny Scotland.
Centre for Cell Engineering,University of Glasgow,UK.
mail: rh at mblab.gla.ac.uk, Tel: ++44 (0)141 330 4756
Web : http://www.gla.ac.uk/Inter/CellEngineering
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