Getting DMSO out of parafin wax?
Bernard Murray, PhD
spam at 127.0.0.1
Mon Mar 20 02:00:49 EST 2000
<Pine.SOL.3.95.1000318132847.679A-100000 at london.biology.lsa.umich.edu>,
Michael Prigge <prigge at biology.lsa.umich.edu> wrote:
> I was going to do some hot-start PCR with parafin. (I.e., having the Taq
> separated from the oligos and DNA by wax that melts during the first
> denaturation step.) Well, I realized that this lab has cases of Paraplast
> Plus parafin containing an unspecified amount of DMSO, but no DMSO-free
> parafin. If I extract the wax a couple times with water, would that get
> rid of enough of the DMSO that I won't have to alter my PCR conditions?
I've not seen any adverse effects of DMSO (eg. 5%) on PCR
results. In fact it usually gives a nice improvement so
unless there is a *lot* of DMSO in the wax or your PCR
conditions are *really* finely tuned I wouldn;t bother removing
it - just test it and see.
Bernard P. Murray, PhD
bpmurray at cgl . ucsf . edu
Department of Cellular & Molecular Pharmacology, UCSF
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