Nuclear/cytoplasmic RNA fractions

Vincent Hurez Vincent.Hurez at ccc.uab.edu
Tue Mar 21 20:15:23 EST 2000


I am trying to isolate nuclear and cytoplasmic RNA from mouse spleen cells.
I tried different detergent lysis condition but the RNA looks degraded after
1min in the detergent. Does anyone has a good reference or protocol for
nuclear RNA extraction from fresh tissue (i.e. spleen) ? And what marker
could I use to assess the purity of my nuclear RNA prep ?


 Thanks in advance for your help.






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