Nuclear/cytoplasmic RNA fractions
Vincent.Hurez at ccc.uab.edu
Tue Mar 21 20:15:23 EST 2000
I am trying to isolate nuclear and cytoplasmic RNA from mouse spleen cells.
I tried different detergent lysis condition but the RNA looks degraded after
1min in the detergent. Does anyone has a good reference or protocol for
nuclear RNA extraction from fresh tissue (i.e. spleen) ? And what marker
could I use to assess the purity of my nuclear RNA prep ?
Thanks in advance for your help.
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