Yellow MOPS - why?

ChenHA hzhen at freeuk.com
Tue Mar 21 21:47:22 EST 2000


"Hiranya S. Roychowdhury" wrote:
> 
> At 04:49 PM 3/21/00 -0000, Wolfgang Schechinger wrote:
> >Hi all,
> >
> >I'd appreciate any explanation why our MOPS buffer  (pH 7, compsition
> >below) got yellow after autoclaving. Is this commonly observed?
> 
> Yes, indeed!
> 
> >(It's not the first time we make this buffer, but the first time it
> >got yellow. Maybe it was heated up a bit too long?)
> 
> Everytime I autoclaved 10xMOPS buffer, it turns a straw color.
> I am not sure why this happened to you for the FIRST time!
> The degree of coloration is also directly proportional to the conc. of the
> MOPS buffer. The coloration in a 1x buffer is barely detectable, while a 20x
> stock turns yellow.
> 

I thought the MOPS buffer should be filter sterilised, not
autoclaved.




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