Kinase inhibitor party-pack?
victorke at duke.edu
Wed Mar 22 04:55:52 EST 2000
Without knowing more detail, it is hard to direct you to the right
direction. Signal transduction pathway is complicated. Looks like
it is receptor related problem. I would not go any further without
checking out a couple of receptors first.
Promega has some kits and kinase inhibitors
you may find useful.
On 14 Mar 2000, Ian A. York wrote:
> Date: 14 MAR 2000 15:04:33 GMT
> From: Ian A. York <iayork at panix.com>
> Newgroups: bionet.cellbiol, bionet.molbio.methds-reagnts
> Subject: Kinase inhibitor party-pack?
> While testing for something altogether different, I found my mutant cells
> are repaired by treatment with genistein, an inhibitor of tyrosine
> kinases. Although there are a couple of obvious candidate substrates,
> specific tests have suggested the obvious ones are not what's mediating
> the effect.
> I'd like to try to narrow down this effect more, to localize which kinase
> (or equally which substrate) is being affectedbut I'm pretty ignorant
> about kinases and signal pathways. Is there a logical, or standard (or
> both) progression for using more selective inhibitors to narrow down the
> Does someone sell a cheap introductory collection of kinase inhibitors,
> suitable for this kind of narrowing down? I'm poking around a number of
> catalogues, and things aren't really coming clear to me.
> Thanks for any tips.
> Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
> "-but as he was a York, I am rather inclined to suppose him a
> very respectable Man." -Jane Austen, The History of England
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