DNA isolation from paraffin-imbedded tissue

Hinrich Schulenburg hschulen at uni-muenster.de
Wed Mar 22 06:55:18 EST 2000

Dear All,

I intend to isolate genomic DNA from ethanol-fixed paraffin-imbedded tissue for subsequent PCR amplifications.
I have a couple of protocols for this purpose (retrieved from various journals), but no experience.

Therefore, I wonder if anyone of you has ever tried to isolate DNA from paraffin-imbedded tissues and could thus advise me on the reliability/suitability of different protocols.

Many thanks in advance,


Hinrich Schulenburg
Abteilung fuer Evolutionsbiologie
Institut fuer Spezielle Zoologie
Westfaelische Wilhelms-Universitaet Muenster
Huefferstr. 1
48149 Muenster
Tel: +49-251-8321019
Fax: +49-251-8324668
e-mail: hschulen at uni-muenster.de
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