Vacuum Blotter - RNA transfer

VASANTHARAJAN JANAKIRAMAN vasant at cc.UManitoba.CA
Thu Mar 23 09:36:09 EST 2000


Hi netters,
We bought a Vacuum transfer system from Pharmacia just a few weeks back.
It works fine for Genomic DNA transfers-for doing southerns, but it
doesn't seem to work for RNA transfers. I run my RTNA in a formaldehyde
gel with 1XMOPS running buffer. I stained the gel with minimal amounts of
EtBr. My transfer buffer was 20xSSC.
Even after 2 hrs of transfer at 55mbar, my RNA (Wheat RNA) was still in
the gel.
I would appreciate any kind of input/advice.
Thanks
Vasanth
My email add.
vasant at plantpath.ksu.edu


---




More information about the Methods mailing list