Oligonucleotides: I'm desperate

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Mar 23 12:50:29 EST 2000

In article <8bdisg$4vgkc$1 at fu-berlin.de>, January Weiner
<nospam_jweiner1 at ix.urz.uni-heidelberg.de> writes
>       Any suggestions? (or maybe I should start working as a taxi
>       driver....)

Make sure you have no ammonium ions in the oligos from de-
protection/EtOH pptation. Inhibitory to T4PNK therfore poor labelling.
Just a thought.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list