Yellow MOPS - why?

Frederik Börnke ricky_boernke at gmx.net
Fri Mar 24 02:55:37 EST 2000




We do always autoclave our solutions and never observed any problems (We
use DEPC treatment).
As far as I understood autoclaving is just to inactivate the DEPC not
the RNAses
(they should be inactivated by DEPC). But anyway, for gel running buffer
we don't care
about anything like autoclaving or RNAes, using formamide and
formaldehyde within the gel
and within the loading buffer, the whole thing happens under denaturing
conditions, so why
bohter?


Ricky


*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de




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