any ideas for substitute for chloroform

Austin P. So (Hae-Jin) haejin at netinfo.ubc.caX
Fri Mar 24 12:36:07 EST 2000


What about the relative recovery? Or relative purity?

I would think that the partitioning of nucleic acids/proteins may be different
in a TCB/CHCl3 mixture...but it may be negligible, so who knows? But it would be
the more important criteria for small sample sizes...

Cheers

Austin

"Hamel, Andre (AGR)" wrote:

>         Last week I rec'd the trichlorobutanol (Sigma-Aldrich cat no
> 11,205-4) and
> tested it on several preps (DNA and RNA from tissues, blood, semen and
> serum).
> They all looked normal and PCRed great (no changes from nucl acid using
> CHCl3).

--
---
Austin P. So (Hae Jin)

I.I.S.G.P.
Biotechnology Laboratory
University of British Columbia

E-mail: haejin at netinfo.ubc.ca

http://www.interchange.ubc.ca/haejin/index.html (under construction)






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